Journal: Technology in cancer research & treatment

Article Title: Fascin Overexpression Promotes Cholangiocarcinoma RBE Cell Proliferation, Migration, and Invasion.

doi: 10.1177/1533034615580696

Figure Lengend Snippet: Figure 4. Fascin overexpression inhibited E-cadherin expression in cholangiocarcinoma RBE cells. A, E-cadherin mRNA expression was detected in each group of cells by real-time PCR. B, E-cadherin was detected by immunofluorescence. Fluorescence microscopy showed that E-cadherin was highly expressed in the cells of parental group and the pcDNA3.1 group (red), and the nuclei were stained blue by DAPI. Scale bars 20 mm. C, E-cadherin protein expression levels were detected by Western blot, and b-actin served as an internal control for the grayscale analysis. The data are presented as the mean + standard deviation. Compared with the pcDNA3.1 transfection group, ** indicates P < .01. Compared with the parental group, ## indicates P < .01. mRNA indicates messenger RNA; PCR, polymerase chain reaction; cDNA, comple- mentary DNA; DAPI, 40,6-diamidino-2-phenylindole.

Article Snippet: The fascin (1:100 dilution, Boster) and E-cadherin antibodies (1:100 dilution, Wanleibio) were added, followed by incubation at 4 C overnight.

Techniques: Over Expression, Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Fluorescence, Microscopy, Staining, Western Blot, Control, Standard Deviation, Transfection, Polymerase Chain Reaction

Journal: Technology in cancer research & treatment

Article Title: Fascin Overexpression Promotes Cholangiocarcinoma RBE Cell Proliferation, Migration, and Invasion.

doi: 10.1177/1533034615580696

Figure Lengend Snippet: Figure 5. The regulation of E-cadherin expression by fascin was independent of NF-kB signaling pathway. A, Cytoplasmic p-IkB and nuclear NF-kB p65 protein expression levels were detected by Western blot, and the grayscale analysis was performed with b-actin and Lamin A as the internal controls, respectively. The data are presented as the mean + standard deviation. B and C, E-cadherin protein expression was detected by Western blot. For the NF-kB inhibitor treatment group, BAY11-7082 was added to a final concentration of 3 mmol/L and incubated for 24 hours before analyzing E-cadherin expression. For the NF-kB siRNA intervention group, cell transfection was performed according to the manufacturer’s instructions. Representative results are shown in this figure. The data are presented as the mean + standard deviation. Compared with the pcDNA3.1 transfection group, ** indicates P < .01. Compared with the parental group, ## indicates P < .01. Compared with the pcDNA3.1þBAY11-7082 group, $$ indicates P < .01. NF-kB indicates nuclear factor-kB; siRNA, small interfering RNA; cDNA, comple- mentary DNA.

Article Snippet: The fascin (1:100 dilution, Boster) and E-cadherin antibodies (1:100 dilution, Wanleibio) were added, followed by incubation at 4 C overnight.

Techniques: Expressing, Western Blot, Standard Deviation, Concentration Assay, Incubation, Transfection, Small Interfering RNA

Journal: Oncology Letters

Article Title: Analyses on K-ras mutations and fascin expression in patients with cardia cancer

doi: 10.3892/ol.2018.9750

Figure Lengend Snippet: Primer sequences of fluorescence quantitative PCR.

Article Snippet: Then, the sections were stained using immunohistochemistry MaxVision two-step method blocked with 5% milk at 20°C for 2 h. After that, mouse anti-human fascin monoclonal antibodies (dilution, 1:200; MAB-0228, clone no. FCN01) and MaxVision immunohistochemistry kits (both from Fuzhou Maxim Biotech, Inc., Fuzhou, China) were used.

Techniques: Fluorescence

Journal: Oncology Letters

Article Title: Analyses on K-ras mutations and fascin expression in patients with cardia cancer

doi: 10.3892/ol.2018.9750

Figure Lengend Snippet: Results of immunohistochemistry for fascin in cardia cancer (p<0.05). Results of immunohistochemistry in (A) the non-mutant and (B) mutant groups. The number of positive cells in the mutant group is greater than that in the non-mutant group (p<0.05).

Article Snippet: Then, the sections were stained using immunohistochemistry MaxVision two-step method blocked with 5% milk at 20°C for 2 h. After that, mouse anti-human fascin monoclonal antibodies (dilution, 1:200; MAB-0228, clone no. FCN01) and MaxVision immunohistochemistry kits (both from Fuzhou Maxim Biotech, Inc., Fuzhou, China) were used.

Techniques: Immunohistochemistry, Mutagenesis

Journal: Oncology Letters

Article Title: Analyses on K-ras mutations and fascin expression in patients with cardia cancer

doi: 10.3892/ol.2018.9750

Figure Lengend Snippet: Results of qPCR. The expression level of fascin gene in the mutant group is higher than that in the non-mutant group. qPCR, quantitative polymerase chain reaction. *p<0.05, compared with the non-mutant group.

Article Snippet: Then, the sections were stained using immunohistochemistry MaxVision two-step method blocked with 5% milk at 20°C for 2 h. After that, mouse anti-human fascin monoclonal antibodies (dilution, 1:200; MAB-0228, clone no. FCN01) and MaxVision immunohistochemistry kits (both from Fuzhou Maxim Biotech, Inc., Fuzhou, China) were used.

Techniques: Expressing, Mutagenesis, Real-time Polymerase Chain Reaction